About Me
I am a molecular biologist with a Ph.D. in Biochemistry from the Indian Institute of Science, where I worked under the guidance of Prof. Sathees C. Raghavan. My doctoral work focused on the mechanisms of double-strand break repair and genome fragility in mammalian mitochondria.
Currently, I am a Research Associate at the Memorial Sloan Kettering Cancer Center in New York, in the laboratory of Dr. Dirk Remus. My postdoctoral research involves dissecting the biochemical and structural basis of DNA replication and genome maintenance.
My research expertise spans gene cloning, protein purification, chromatin immunoprecipitation (ChIP), fluorescence techniques and advanced biochemical assays for DNA repair and replication. I am particularly passionate about understanding how endogenous factors influence genomic stability and contribute to disease.
I have authored several peer-reviewed publications in journals like iScience, eLife, The FEBS Journal, and Journal of Biological Chemistry, and have presented at leading conferences including the ASCB-EMBO Meeting. I aim to bridge fundamental research in genome biology with translational insights into disease mechanisms.
Research Skills: Protein Purification, ChIP, Gene Cloning, Cell Culture, DNA Replication Assays
[Selected Papers] [Full List] [Google Scholar] [ResearchGate]
Selected Papers:
- Homologous recombination-mediated repair of DNA double-strand breaks operates in mammalian mitochondria
Dahal, S., Dubey, S., & Raghavan, S.C.
Cellular and Molecular Life Sciences (2018) - G-quadruplex Structures Contribute to Differential Radiosensitivity of the Human Genome
Kumari, N., Vartak, S.V., Dahal, S., Kumari, S., Desai, S.S., Gopalakrishnan, V., et al.
iScience (2019) - Mitochondrial genome stability in human: understanding the role of DNA repair pathways
Dahal, S., & Raghavan, S.C.
Biochemical Journal (2021) - Characterization of G4 DNA formation in mitochondrial DNA and their potential role in mitochondrial genome instability
Dahal, S., Siddiqua, H., Katapadi, V.K., Iyer, D., & Raghavan, S.C.
The FEBS Journal (2022) - Unleashing a novel function of endonuclease G in mitochondrial genome instability
Dahal, S., Siddiqua, H., Sharma, S., Babu, R.K., Rathore, D., Sharma, S., et al.
eLife (2022)